Prof. Emanuele Domenico Giordano

• The slides presented in classes will be available @ IOL.

Argomenti del programma: - Setting a research protocol: instruments and reagents in a biomedical laboratory. - Setting a research protocol: cell cultures. - Imaging and counting cells: principles of optical microscopy (bright field, phase contrast). - Molecular components of a cell: aminoacids and proteins in solution. - UV and visible spectroscopy to rapidly determine nucleic acid or protein concentrations.

• Slides from classes will be available.

Argomenti del programma: Unconventional applications for nucleic acids, e.g. a) DNA nanodevices; b) DNA computing; c) autonomous molecular computers for logical control of gene expression. What is synthetic biology? The international Genetically Engineered Machine competition. The Registry: a promoter and a terminator for transcription, a ribosome binding site (RBS) for translation. Small regulatory RNAs. The lab room and the equipment.

• The slides presented in classes will be available @ IOL.

Argomenti del programma: - Setting a research protocol: instruments and reagents in a biomedical laboratory. - Setting a research protocol: cell cultures. - Imaging and counting cells: principles of optical microscopy (bright field, phase contrast). - Molecular components of a cell: aminoacids and proteins in solution. - UV and visible spectroscopy to rapidly determine nucleic acid or protein concentrations.