Biophotonics (C.I.) – Politecnico di Milano

Argomenti del programma: Optical microscopy and nanoscopy: Propagation of light in an optical microscope: point spread function in an optical microscope and 3D imaging theory. Classical microscopy techniques for cellular and tissue imaging: bright-field, darkfield, phase contrast, fluorescence. Three-dimensional imaging of tissues: confocal microscopy, structured illumination microscopy, 2-photon microscopy.