Prof. Giovanni Nava

• On the course website you will find the files of the slides used in the lectures and a selection of review articles on topics drawn from important international magazines.

Argomenti del programma: ADVANCED OPTICAL MICROSCOPY Introduction on the resolution of optical microscopes Effects of the finite resolution on images Introduction to confocal microscopy Optical processes and techniques that can overcome the resolution limit, such as: non linear microscopy, STED,TIRF,PALM, SNOM. Introduction to time resolved fluorescence and FLIM microscopy NANOTCHNOLOGY Aims and uses of nanoparticles in biomedicine.

• The teaching does not have a dedicated book and all the material will be provided by teachers. All the PowerPoint material used for the lectures will be made available to the students at the dedicated Ariel website.

Argomenti del programma: The teaching will provide the conceptual, technical and computational foundations required to design, perform and quantitatively analyse imaging experiments in living cells. Particular emphasis will be placed on selecting the most appropriate imaging approach to address specific biological questions and on extracting quantitative information from microscopy data.

• Slides of the lessons available on the website of the course

Argomenti del programma: The topics are: - Description of the dynamics of biomolecules in terms of fundamental interactions and diffusive motion. - Thermodynamic equilibrium of the biomolecular conformations and interactions. - Thermodynamics of DNA hybridization, protein folding and biomolecular recognition processes.